Reporter

Part:BBa_K3026012:Design

Designed by: Pascale Lemieux   Group: iGEM19_ULaval   (2019-10-16)


Toehold norovirus ORF2 VP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 167


Design Notes

ToeHold were designed with sequence constraints according to the engineered design from Green et al. 2014. sfGFP was chosen according to its expression profile in cell-free media containing T7 polymerase, and according to its excitation and emission spectra.


Source

Synthetic construct. Switch sequence comes from the reference genome of norovirus GII/Hu/JP/2007/GII.P15_GII.15/Sapporo/HK299. sfGFP sequence comes from part BBa_K1321337. Other ToeHold sequence come from Green et all 2014 Supp. Data switch #1

References